While many studies on the depiction of queer characters focused mainly on American television shows, not much research has been done on depictions of queer characters in alternative mediums. Japan has two distinct genres that focus on homosexuality: Yaoi and Yuri. Yaoi loosely refers to homosexual relations between males while Yuri loosely refers to homosexual relations between females. This article, however, will be focusing exclusively on the depiction of lesbian characters in anime, with Aoi Hana being used as a case study.
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Oniisama E actually ranks lower on this multi-person synthesised list than it does on my personal list where it comes in at 5. The series centers around the high school experiences of Misonou Nanako note: for this and subsequent names, family name comes first when she enters the elite preparatory school, Seiran Academy. For those who might be expecting overt romance, however, they'll be disappointed. As Yuricon 's Erica Friedman points out, the series deals more with akogare yearning than it does with actual romance. There is a great deal of akogare in this series, and very little outright Yuri, but I'm going to have to weigh in on the yes, Yuri camp.